《植物生理学报》 2018, 54(5): 812-818

K⁺/Na⁺浓度比对罗汉果悬浮细胞生长和甜苷V合成的影响

陈雨霞¹, 王泽建¹, 肖慈英¹, 庄英萍^1,2, 宋云飞³, 郭美锦^1,2,*

¹华东理工大学生物反应器工程国家重点实验室, 上海200237; ²上海生物制造技术协同创新中心, 上海200237; ³桂林莱茵生物科技股份有限公司, 广西桂林541199

通信作者：郭美锦；E-mail: guo_mj@ecust.edu.cn

摘　要：

本文研究了不同K⁺/Na⁺浓度比对罗汉果悬浮细胞生长和甜苷V合成的影响。结果表明, 合适的K⁺/Na⁺浓度比促进细胞生长和甜苷V生物合成。当K⁺/Na⁺最佳浓度比为15:1时, 罗汉果悬浮细胞内K+浓度、K⁺/Na⁺、S_{(K, Na)}值均为最大, 此时细胞利用K⁺效率最高, 细胞生长速率最快。此外, 胞外有机酸和胞内氨基酸的变化表明此浓度比例下, 细胞可能通过降低TCA循环代谢强度, 以及增强EMP途径和MVA途径代谢强度, 从而促进萜类化合物前体乙酰CoA向罗汉果甜苷V合成。

关键词：罗汉果; 悬浮培养; K⁺/Na⁺浓度比; 甜苷V生物合成

收稿：2018-03-01   修定：2018-04-28

资助：国家科技重大专项(2017ZX07402003)。

Physiologically affects of ratio of K⁺ to Na⁺ on cell growth and mogrosides V biosynthesis in Siraitia grosvenorii cells cultivated in suspension culture

CHEN Yu-Xia¹, WANG Ze-Jian¹, XIAO Ci-Ying¹, ZHUANG Ying-Ping^1,2, SONG Yun-Fei³, GUO Mei-Jin^1,2,*

¹State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; ²Shanghai Biological Manufacturing Technology Innovation Center, Shanghai 200237, China; ³Guilin Layn Biotechnology Company, Guilin, Guangxi 541199, China

Corresponding author: GUO Mei-Jin; E-mail: guo_mj@ecust.edu.cn

Abstract:

The effect of K⁺/Na⁺ ratios on the cell growth and mogrosides V production in suspended Siraitia grosvenorii cells culture were investigated in this paper. The results showed that an appropriate ratio of K⁺ to Na⁺ could significantly improve the cell growth as well as mogrosides V biosynthesis. Under the optimal condition of at 15:1, the intracellular K⁺_cell, K⁺/Na⁺ ratio and S_{(K, Na)} were observed at their maximum values, simultaneously, the highest K⁺ uptaken rate and cell growth rate were also obtained. Moreover, through the extracellular organic acid and amino acid profiles it was inferred that at the level of K⁺/Na⁺ ratio of 15:1, the decrease of TCA metabolic flux as well as the increases of EMP metabolic flux and MVA metabolic flux could lead to the precursor acetyl CoA utilization for mogrosides V production.

Key words: Siraitia grosvenorii; suspension culture; K⁺/Na⁺ concentration ratio; mogrosides V biosynthesis